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| Registros recuperados : 9 | |
| 1. |  | PINTO, P. P.; RAPOSEIRAS, R.; MACEDO, A. M.; SELDIN, L.; PAVA, E.; SA, N. M. H. Effects of high temperature on survival, symbiotic performance and genomic modifications of bean nodulating Rhizobium strains. Revista de Microbiologia, São Paulo, v. 29, n. 4, p. 295-300, 1998.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | FERREIRA, C. R.; FANTINI JUNIOR, M.; COLODETTE, J. L.; GOMIDE, J. L.; MACEDO, A. M.; CARVALHO, A. M. M. L. Avaliação tecnológica de clones de eucalipto: parte 1 - qualidade da madeira para produção de celulose Kraft. Scientia Forestalis, São Paulo, n. 70, p. 161-170, abr. 2006.| Biblioteca(s): Embrapa Florestas. |
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| 6. | | MOURÃO, M. M.; BITAR, M. L.; LOBO, F. P.; GRYNBERG, P.; BORONI, M.; WAISBERG, M.; MACEDO, A. M.; MACHADO, C. R.; FRANCO, G. R. Revealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Shistosoma mansoni. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011. Não paginado. X-MEETING 2011.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 7. | | MOURÃO, M. de M.; BITAR, M.; LOBO, F. P.; PECONICK, A. P.; GRYNBERG, P.; PROSDOCIMI, F.; WAISBERG, M.; CERQUEIRA, G. C.; MACEDO, A. M.; MACHADO, C. R.; YOSHINO, T.; FRANCO, G. R. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 8. | | BITAR, M.; DRUMMOND, M. G.; COSTA, M. G. S.; LOBO, F. P.; CALZAVARA-SILVA, C. E.; BISCH, P. M.; MACHADO, C. R.; MACEDO, A. M.; PIERCE, R. J.; FRANCO, G. R. Modeling the zing finger protein SmZF1 from Schistosoma mansoni: Insights into DNA binding and gene regulation. Journal of Molecular Graphics and Modelling, New York, v. 39, p. 29-38, 2013.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 9. | | VIEIRA, H. G. S. V.; GRYNBERG, P.; BITAR, M.; PIRES, S. da F.; HILÁRIO, H. O.; MACEDO, A. M.; MACHADO, C. R.; ANDRADE, H. M. de; FRANCO, G. R. Proteomic analysis of Trypanosoma cruzi response to ionizing radiation stress. Cell Reports, v. 9, p. 378-390, 2014. (Open Acess)| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 9 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
14/02/2013 |
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Data da última atualização: |
08/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BITAR, M.; DRUMMOND, M. G.; COSTA, M. G. S.; LOBO, F. P.; CALZAVARA-SILVA, C. E.; BISCH, P. M.; MACHADO, C. R.; MACEDO, A. M.; PIERCE, R. J.; FRANCO, G. R. |
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Afiliação: |
MAINÁ BITAR, UFRJ, UFMG; MARCELA GONÇALVES DRUMMOND, UFMG, Univ. Lille Nord de France; MAURICIO GARCIA SOUZA COSTA, UFRJ; FRANCISCO PEREIRA LOBO, CNPTIA; CARLOS EDUARDO CALZAVARA-SILVA, Fiocruz; PAULO MASCARELLO BISCH, UFRJ; CARLOS RENATO MACHADO, UFMG; ANDRÉA MARA MACEDO, UFMG; RAYMOND J. PIERCE, Univ. Lille Nord de France; GLÓRIA REGINA FRANCO, UFMG. |
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Título: |
Modeling the zing finger protein SmZF1 from Schistosoma mansoni: Insights into DNA binding and gene regulation. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Molecular Graphics and Modelling, New York, v. 39, p. 29-38, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Zinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites revealed 415 genes hypothetically under SmZF1 control. Using an automatic annotation and GO assignment approach, we found that the majority of those genes code for proteins involved in developmental processes. Taken together, these results present a consistent base to the structural and functional characterization of SmZF1. MenosZinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites reve... Mostrar Tudo |
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Palavras-Chave: |
Ligação de DNA; Regulação gênica. |
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Thesagro: |
Schistosoma Mansoni. |
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Thesaurus NAL: |
DNA-binding proteins; Gene expression regulation; Zinc finger motif. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aBITAR, M.
245 $aModeling the zing finger protein SmZF1 from Schistosoma mansoni$bInsights into DNA binding and gene regulation.$h[electronic resource]
260 $c2013
520 $aZinc finger proteins are widely found in eukaryotes, representing an important class of DNA-binding proteins frequently involved in transcriptional regulation. Zinc finger motifs are composed by two antiparallel b-strands and one a-helix, stabilized by a zinc ion coordinated by conserved histidine and cysteine residues. In Schistosoma mansoni, these regulatory proteins are known to modulate morphological and physiological changes, having crucial roles in parasite development. A previously described C2H2 zinc finger protein, SmZF1, was shown to be present in cell nuclei of different life stages of S. mansoni and to activate gene transcription in a heterologous system. A high-quality SmZF1 tridimensional structure was generated using comparative modeling. Molecular dynamics simulations of the obtained structure revealed stability of the zinc fingers motifs and high flexibility on the terminals, comparable to the profile observed on the template X-ray structure based on thermal b-factors. Based on the protein tridimensional features and amino acid composition, we were able to characterize four C2 H2 zinc finger motifs, the first involved in protein?protein interactions while the three others involved in DNA binding. We defined a consensus DNA binding sequence using three distinct algorithms and further carried out docking calculations, which revealed the interaction of fingers 2?4 with the predicted DNA. A search for S. mansoni genes presenting putative SmZF1 binding sites revealed 415 genes hypothetically under SmZF1 control. Using an automatic annotation and GO assignment approach, we found that the majority of those genes code for proteins involved in developmental processes. Taken together, these results present a consistent base to the structural and functional characterization of SmZF1.
650 $aDNA-binding proteins
650 $aGene expression regulation
650 $aZinc finger motif
650 $aSchistosoma Mansoni
653 $aLigação de DNA
653 $aRegulação gênica
700 1 $aDRUMMOND, M. G.
700 1 $aCOSTA, M. G. S.
700 1 $aLOBO, F. P.
700 1 $aCALZAVARA-SILVA, C. E.
700 1 $aBISCH, P. M.
700 1 $aMACHADO, C. R.
700 1 $aMACEDO, A. M.
700 1 $aPIERCE, R. J.
700 1 $aFRANCO, G. R.
773 $tJournal of Molecular Graphics and Modelling, New York$gv. 39, p. 29-38, 2013.
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